Select the first ROI from the list in the ROI Manager, then open the 'File' menu, 'Save As' and select 'XY Coordinates.'.
You can use the polygonal tool to achieve is similar point-to-point shape if that is easier to use.
The coordinates of the ROI polygon can then be exported, but please pay attention to the names you give each ROI (as per instructions, below). Using ImageJ you can draw ROIs on pixel-based images.
Imagej software script install#
You can download Fiji here and follow the instructions to install it to your computer. Fiji is also maintained and updated more frequently and for this reason ImageJ recommends downloading Fiji. Fiji is a distribution of ImageJ, so it includes 'plain' ImageJ together with many useful community-maintained tools and plugins. ImageJ is a popular open-source program for image processing and analysis.